Summary of Técnicas de ADN recombinante

00:00:00 - 00:10:00

This video discusses the various techniques that can be used to manipulate DNA, with a focus on how these techniques can be used to create new gene sequences. These techniques are based on the properties of DNA that allow it to be cut and joined in specific ways, and they have been used to great effect in the field of genetic engineering.

• 00:00:00 Mis colleagues and professor José Guadalupe Gracias Sánchez with me are going to talk about the techniques of recombinant DNA on the topic of gene manipulation. In the early years of the 1970s, technologies for manipulating genes were developed. Techniques of recombinant DNA are based on a few properties of DNA that have allowed progress to be made very rapidly over the years, starting in the 1970s. One of the basic properties of DNA is that it is a double strand, and the complementary bases of two strands can be joined together to form a double strand. This is the basis of most gene manipulation techniques. Another property of DNA is that it codes for something, and it can produce that protein if it is placed in the right conditions. Enzymes that are known as restriction enzymes can cut DNA at specific places and have the advantage of being able to cut DNA in specific places. These enzymes were discovered in bacteria in the 1970s and have since been found in other organisms. By understanding the sequence of the restriction enzyme, it is possible to design gene constructs that are 8-10 bases long but have to be organized in an exact sequence. One of the early achievements of recombinant DNA technology was the creation
• 00:05:00 This video outlines various techniques for recombinant DNA, or DNA that has been manipulated in some way. One of the purposes of such manipulation is to allow a plasmid to naturally spread among bacteria, which can then be used to create mutants. If a piece of DNA is amplified and copies of it are obtained quickly, we say that the piece of DNA has been cloned. To clone a full genome, however, large pieces of DNA must be used. One of the first steps in this process was to use viruses as vectors, and then other techniques were developed that combined pieces of plasmid with chromosomes from different organisms. As technology advances, it is becoming easier and easier to clone entire genomes.
• 00:10:00 This video demonstrates the use of recombinant DNA techniques to create new gene sequences.